Journal: bioRxiv
Article Title: Acute and Lifelong Exercise Modulate the Tumorigenic Potential of Human Lung Cancer Cells and Their Susceptibility to Cisplatin
doi: 10.64898/2026.03.19.713009
Figure Lengend Snippet: Neither acute nor lifelong exercise serum conditioning affected the in vitro migratory capacity of A549 human lung cancer cells. ( A–C ) To assess in vitro migratory capacity, two artificial 500 µm cell-free gaps were created in confluent monolayer cultures, using 3-well culture inserts that were placed onto the wells of 24-well plates, one insert per well and per serum tested. Cultures were then exposed to medium supplemented with 10% (v/v) human serum. For each gap, three different regions were imaged immediately after its creation and after an 18-hour incubation. Cell-free areas in these images were then measured using ImageJ and the percentage of gap closure over this period was calculated. For each serum, the average of the corresponding six values was then used as an estimate of cell migration potential. Dots and squares represent individual values for the different sera. Each set of connected dots represents baseline (left) and post-intervention (right) sera from the same participant. Large horizontal bars and associated smaller horizontal (error) bars represent means ± standard deviation for the 13 participants of each cohort. The statistical significance of the effect of lifelong exercise was assessed using the Mann-Whitney test. All other comparisons were made by repeated measures two-way ANOVA, assuming sphericity. Statistical significance was set at P < 0.05 and the null hypothesis was never rejected (ns). Results are from a single independent experiment in which each serum was tested in two artificial gaps. ( D ) Representative micrographs (40× magnification) of artificial gaps for each of the four conditions tested, taken immediately after the creation of the gap and after an 18-h incubation in growth medium supplemented with 10% (v/v) human serum. Micrographs were captured using an Olympus CKX53 inverted optical microscope equipped with a camera and the EPview™ software (V2.9.6_20201224; Hachioji, Tokyo, Japan).
Article Snippet: All images were captured with a 40× magnification, using an Olympus CKX53 inverted optical microscope equipped with a camera and the EPviewTM software (V2.9.6_20201224; Hachioji, Tokyo, Japan).
Techniques: In Vitro, Incubation, Migration, Standard Deviation, MANN-WHITNEY, Microscopy, Software
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